Bioss abcb9 polyclonal antibody
Abcb9 Polyclonal Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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abcb9 antibody (Bio-Techne corporation)

Bio-Techne corporation abcb9 antibody
Abcb9 Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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abcb9 (Proteintech)

Proteintech abcb9
Abcb9, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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abcb9 (Santa Cruz Biotechnology)

Santa Cruz Biotechnology abcb9

Figure 6. Reintroduction of miR-31 Affects Lysosomal Drug Transport (A) Expression levels of the drug inﬂux transporter <t>abcb9</t> were analyzed via qPCR. There is a signiﬁcantly greater relative expression level (p = 0.0251) of ABCB9 in miR-31- transfected cells compared to the miR-VC-transfected equivalent (n = 4). RQ relates to relative fold change. (B) Representative western blot illustrating an increase in ABCB9 expression level with miR-31 re-expression, with no apparent change in the lysosomal marker LAMP1. (C) Densitometry analysis revealing signiﬁcant (p = 0.0325) upre- gulation of ABCB9 (n = 3). (D) Mean signal intensities of NCI-H2452-transfected cells immunoﬂuorescently stained with ABCB9 or LAMP1, with intensities captured in the Texas red channel for the images in (E). (E and F) Immunoﬂuorescent images showing slight alteration in ABCB9 (E) or LAMP1 (F) staining (red); nuclei are stained with DAPI (blue). Images were captured on LSM 710 with a 63/1.40 oil DIC M27 objective for ABCB9 images and a 10/0.25 Ph1 objective for LAMP1 images. Data are presented as the mean ± SEM. *p < 0.05; **p < 0.01.

Abcb9, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results

Figure 6. Reintroduction of miR-31 Affects Lysosomal Drug Transport (A) Expression levels of the drug inﬂux transporter abcb9 were analyzed via qPCR. There is a signiﬁcantly greater relative expression level (p = 0.0251) of ABCB9 in miR-31- transfected cells compared to the miR-VC-transfected equivalent (n = 4). RQ relates to relative fold change. (B) Representative western blot illustrating an increase in ABCB9 expression level with miR-31 re-expression, with no apparent change in the lysosomal marker LAMP1. (C) Densitometry analysis revealing signiﬁcant (p = 0.0325) upre- gulation of ABCB9 (n = 3). (D) Mean signal intensities of NCI-H2452-transfected cells immunoﬂuorescently stained with ABCB9 or LAMP1, with intensities captured in the Texas red channel for the images in (E). (E and F) Immunoﬂuorescent images showing slight alteration in ABCB9 (E) or LAMP1 (F) staining (red); nuclei are stained with DAPI (blue). Images were captured on LSM 710 with a 63/1.40 oil DIC M27 objective for ABCB9 images and a 10/0.25 Ph1 objective for LAMP1 images. Data are presented as the mean ± SEM. *p < 0.05; **p < 0.01.

Journal: Molecular therapy. Nucleic acids

Article Title: MicroRNA-31 Regulates Chemosensitivity in Malignant Pleural Mesothelioma.

doi: 10.1016/j.omtn.2017.07.001

Figure Lengend Snippet: Figure 6. Reintroduction of miR-31 Affects Lysosomal Drug Transport (A) Expression levels of the drug inﬂux transporter abcb9 were analyzed via qPCR. There is a signiﬁcantly greater relative expression level (p = 0.0251) of ABCB9 in miR-31- transfected cells compared to the miR-VC-transfected equivalent (n = 4). RQ relates to relative fold change. (B) Representative western blot illustrating an increase in ABCB9 expression level with miR-31 re-expression, with no apparent change in the lysosomal marker LAMP1. (C) Densitometry analysis revealing signiﬁcant (p = 0.0325) upre- gulation of ABCB9 (n = 3). (D) Mean signal intensities of NCI-H2452-transfected cells immunoﬂuorescently stained with ABCB9 or LAMP1, with intensities captured in the Texas red channel for the images in (E). (E and F) Immunoﬂuorescent images showing slight alteration in ABCB9 (E) or LAMP1 (F) staining (red); nuclei are stained with DAPI (blue). Images were captured on LSM 710 with a 63/1.40 oil DIC M27 objective for ABCB9 images and a 10/0.25 Ph1 objective for LAMP1 images. Data are presented as the mean ± SEM. *p < 0.05; **p < 0.01.

Article Snippet: Proteins were separated on 7.5% or 10% SDS-PAGE gels, transferred onto polyvinylidene fluoride (PVDF) membrane (Thermo Fisher Scientific), and probed for CTR1 (sc-66847) used at 1:1,000, ABCB9 (sc-393412) used at 1:1,000, LAMP1 (sc-17768) used at 1:1,000, OCT1 (sc-293181) used at 1:1,000, b-actin (sc-130300) used at 1:10,000 (Santa Cruz Biotechnology), and gH2A.X (9718) used at 1:1,000 (Cell Signal), followed by incubation with anti-mouse horseradish peroxidase (HRP)-conjugated secondary antibody (P0260) used at 1:2,000 (Dako) or anti-rabbit HRP-conjugated secondary antibody (sc-2004) used at 1:2,000 (Santa Cruz Biotechnology).

Techniques: Drug Transport Assay, Expressing, Transfection, Western Blot, Marker, Staining

Figure 7. The Bipotential Transcriptional Regulator, OCT1, Can Be Associated with miR-31 Expression (A) Representative western blot illustrating the downregulation of a potential nega- tive regulator of both CTR1 and ABCB9 expression, OCT1, with miR-31 re- introduction. Suppression of miR-31 does not affect expression of OCT1. (B) Densitometry analysis of OCT1 expression with miR-31 reintroduction (NCI- H2452) or miR-31 suppression (P31) (n = 2). The data demonstrate a large decrease in OCT1 with miR-31 re-expression, potentiating miR-31-mediated downregulation of OCT1 and leading to upregulation of CTR1 and ABCB9 expression. Data are presented as the mean ± SEM.

Journal: Molecular therapy. Nucleic acids

Article Title: MicroRNA-31 Regulates Chemosensitivity in Malignant Pleural Mesothelioma.

doi: 10.1016/j.omtn.2017.07.001

Figure Lengend Snippet: Figure 7. The Bipotential Transcriptional Regulator, OCT1, Can Be Associated with miR-31 Expression (A) Representative western blot illustrating the downregulation of a potential nega- tive regulator of both CTR1 and ABCB9 expression, OCT1, with miR-31 re- introduction. Suppression of miR-31 does not affect expression of OCT1. (B) Densitometry analysis of OCT1 expression with miR-31 reintroduction (NCI- H2452) or miR-31 suppression (P31) (n = 2). The data demonstrate a large decrease in OCT1 with miR-31 re-expression, potentiating miR-31-mediated downregulation of OCT1 and leading to upregulation of CTR1 and ABCB9 expression. Data are presented as the mean ± SEM.

Techniques: Expressing, Western Blot

Figure 8. Overexpression of the Lysosomal Drug Transporter ABCB9 Affects Chemosensitivity, Independent of miR-31 (A) Representative western blot demonstrating the increased levels of gH2A.X with high expression of ABCB9 in the parent NCI-H2452 cell line (the ABCB9 over- expression cell line is termed NCI-H2452 ABCB9+). Clones B2–B4 correlate to EX- NEG vector control clones (vector control for ABCB9 overexpression model is termed NCI-H2452 EX-NEG). Clones C2 and C3 correlate to high expressers of ABCB9, and C4 is a low expresser of ABCB9. All clones were treated for 24 hr with 50 mM cisplatin. (B) ABCB9 overexpression sensitizes miR-31 null NCI-H2452 cells to cisplatin after treatment with 1 mM cisplatin for 24 hr, with near signiﬁcance reached (p = 0.0516) (n = 3). Data are presented as the mean ± SEM.

Journal: Molecular therapy. Nucleic acids

Article Title: MicroRNA-31 Regulates Chemosensitivity in Malignant Pleural Mesothelioma.

doi: 10.1016/j.omtn.2017.07.001

Figure Lengend Snippet: Figure 8. Overexpression of the Lysosomal Drug Transporter ABCB9 Affects Chemosensitivity, Independent of miR-31 (A) Representative western blot demonstrating the increased levels of gH2A.X with high expression of ABCB9 in the parent NCI-H2452 cell line (the ABCB9 over- expression cell line is termed NCI-H2452 ABCB9+). Clones B2–B4 correlate to EX- NEG vector control clones (vector control for ABCB9 overexpression model is termed NCI-H2452 EX-NEG). Clones C2 and C3 correlate to high expressers of ABCB9, and C4 is a low expresser of ABCB9. All clones were treated for 24 hr with 50 mM cisplatin. (B) ABCB9 overexpression sensitizes miR-31 null NCI-H2452 cells to cisplatin after treatment with 1 mM cisplatin for 24 hr, with near signiﬁcance reached (p = 0.0516) (n = 3). Data are presented as the mean ± SEM.

Techniques: Over Expression, Western Blot, Expressing, Clone Assay, Plasmid Preparation, Control

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